Journal
JOURNAL OF ZOO AND WILDLIFE MEDICINE
Volume 46, Issue 2, Pages 335-338Publisher
AMER ASSOC ZOO VETERINARIANS
DOI: 10.1638/2013-0293R1.1
Keywords
Aquila chrysaetus; cryopreservation; ejaculate; golden eagle; semen; spermatozoa
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This paper describes the sperm characteristics and response to cooling and freezing of naturally ejaculated semen from a captive, adult golden eagle (Aquila chrysaetus) trained to allow sperm recovery via cooperative copulation. A basic spermiogram was prepared, and sperm motility and morphometric variables recorded using a computer-aided system. For sperm storage, the effects of a polyvinylpyrrolidone-based extender were evaluated at 5 degrees C. The same extender was also used in freezing procedures in which glycerol (11%) and dimethylacetamide (6%) were compared as cryoprotectants. The extender preserved sperm viability over storage periods of up to 6 days. Although sperm motility and percentage live sperm values were poorer for frozen-thawed (5.8-14.6% and 44-42%, respectively) than for fresh samples (46.7 and 74.6%, respectively), no differences were seen between the effects of the two cryoprotectants. These results could be of use when attempting to store the sperm of golden eagles and other raptors.
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