Journal
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
Volume 16, Issue 2, Pages 123-130Publisher
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1400107
Keywords
Moso bamboo; Pb; Phytoremediation; Scanning electron microscopy; Transmission electron microscopy
Categories
Funding
- National Natural Science Foundation of China [31300520]
- Science and Technology Program of Zhejiang Province [2014C33043]
- Zhejiang Provincial Natural Science Foundation of China [LY12C16004]
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A hydroponics experiment was aimed at identifying the lead (Pb) tolerance and phytoremediation potential of Moso bamboo (Phyllostachys pubescens) seedlings grown under different Pb treatments. Experimental results indicated that at the highest Pb concentration (400 mu mol/L), the growth of bamboo seedlings was inhibited and Pb concentrations in leaves, stems, and roots reached the maximum of 148.8, 482.2, and 4282.8 mg/kg, respectively. Scanning electron microscopy revealed that the excessive Pb caused decreased stomatal opening, formation of abundant inclusions in roots, and just a few inclusions in stems. The ultrastructural analysis using transmission electron microscopy revealed that the addition of excessive Pb caused abnormally shaped chloroplasts, disappearance of endoplasmic reticulum, shrinkage of nucleus and nucleolus, and loss of thylakoid membranes. Although ultrastructural analysis revealed some internal damage, even the plants exposed to 400 mu mol/L Pb survived and no visual Pb toxicity symptoms such as necrosis and chlorosis were observed in these plants. Even at the highest Pb treatment, no significant difference was observed for the dry weight of stem compared with controls. It is suggested that use of Moso bamboo as an experimental material provides a new perspective for remediation of heavy metal contaminated soil owing to its high metal tolerance and greater biomass.
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