4.1 Article

Prevalence and Genotype Identification of Toxoplasma gondii in Wild Animals from Southwestern Spain

Journal

JOURNAL OF WILDLIFE DISEASES
Volume 51, Issue 1, Pages 233-238

Publisher

WILDLIFE DISEASE ASSOC, INC
DOI: 10.7589/2013-09-233

Keywords

Genotypes; PCR; PCR-RFLP; Spain; Toxoplasma gondii; wild animals

Funding

  1. Spanish Ministry of Science and Innovation [PI 10/01240, PI 13/01106]
  2. Sixth National Program of I+D+I ISCIII-RICET [RD 12/0018/0011]
  3. Regional Government of Extremadura [PRI08A102]
  4. Department of Employment and Innovation of the Regional Government of Extremadura [PO12010]
  5. European Social Fund

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We used PCR to detect Toxoplasma gondii in the principal game species in southwestern Spain. We detected T. gondii in 32.2% of animals tested. Prevalences varied from 14.7% in wild boar (Sus scrota) to 51.2% in red fox (Vulpes vulpes). The most prevalent genotype was type II (50.0%), followed by type III (20.6%) and type I (5.9%). Mixed infections (11.8%) were detected in wild boar (types I+III) and red fox (types II+III). Polymorphic strains (11.8%) were detected in several species. The high prevalence and the genetic variability shown could have implications for infection of farm animals and humans.

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