Journal
JOURNAL OF WATER AND HEALTH
Volume 13, Issue 1, Pages 210-216Publisher
IWA PUBLISHING
DOI: 10.2166/wh.2014.093
Keywords
Cyclospora cayetanensis; HRM; Italy; real-time PCR; tap water; train
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Tap water samples from the toilets of an Italian national railway train were collected over a period of 10 months and tested for the presence of Cyclospora cayetanensis (C. cayetanensis) using EvaGreen (R) real-time polymerase chain reaction (RT-PCR) assay coupled with high resolution melting (HRM) analysis for protozoan detection and oocyst quantification. C. cayetanensis positive samples were detected in March, April, and May 2013, with the number of oocysts of 4, 5, and 11 per liter, respectively. This is the first finding of C. cayetanensis in water samples in Italy. The findings call for an improvement of hygiene and water safety by the Italian national railway company.
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