Journal
GENOMICS
Volume 92, Issue 4, Pages 210-218Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2008.05.013
Keywords
comparative mapping; macaque and human genomes, rhesus macaque; RH map, radiation hybrid
Funding
- NIH-NCRR [R24 RR017584]
- NATIONAL CENTER FOR RESEARCH RESOURCES [R24RR017584] Funding Source: NIH RePORTER
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A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly. (C) 2008 Elsevier Inc. All Fights reserved.
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