Journal
GENOME BIOLOGY AND EVOLUTION
Volume 6, Issue 1, Pages 247-259Publisher
OXFORD UNIV PRESS
DOI: 10.1093/gbe/evu008
Keywords
intergeneric hybridization; polyploidization; GISH; genome changes; Asteraceae
Categories
Funding
- National Natural Science Foundation of China [31071820, 31071825, 31272203, 31272196]
- 863 project [2011AA100208]
- Fundamental Research Funds for the Central Universities [KYZ201112, KYZ201147]
- University Program for New Century Excellent Talents in of Chinese Ministry of Education [NCET-10-0492, NCET-12-0890]
- Youth Science and Technology Innovation Fund [KJ2011009]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Ask authors/readers for more resources
The Asteraceae family is at the forefront of the evolution due to frequent hybridization. Hybridization is associated with the induction of widespread genetic and epigenetic changes and has played an important role in the evolution of many plant taxa. We attempted the intergeneric cross Chrysanthemum morifolium x Leucanthemum paludosum. To obtain the success in cross, we have to turn to ovule rescue. DNA profiling of the amphihaploid and amphidiploid was investigated using amplified fragment length polymorphism, sequence-related amplified polymorphism, start codon targeted polymorphism, and methylation-sensitive amplification polymorphism (MSAP). Hybridization induced rapid changes at the genetic and the epigenetic levels. The genetic changes mainly involved loss of parental fragments and gaining of novel fragments, and some eliminated sequences possibly from the noncoding region of L. paludosum. The MSAP analysis indicated that the level of DNA methylation was lower in the amphiploid (similar to 45%) than in the parental lines (51.5-50.6%), whereas it increased after amphidiploid formation. Events associated with intergeneric genomic shock were a feature of C. morifolium x L. paludosum hybrid, given that the genetic relationship between the parental species is relatively distant. Our results provide genetic and epigenetic evidence for understanding genomic shock in wide crosses between species in Asteraceae and suggest a need to expand our current evolutionary framework to encompass a genetic/epigenetic dimension when seeking to understand wide crosses.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available