Identification of new winter wheat - winter barley addition lines (6HS and 7H) using fluorescence in situ hybridization and the stability of the whole 'Martonvasari 9 kr1' - 'Igri' addition set

A previous paper reported the development of disomic addition lines (2H, 3H, 4H, and 1HS isochromosomic) from hybrids between the winter wheat 'Martonvasari 9 kr1' and the two-rowed winter barley cultivar 'Igri'. The present paper describes the isolation of two new additions, the 7H disomic and 6HS ditelosomic additions, using fluorescence in situ hybridization with the repetitive DNA probes Afa-family and HvT01. The identification of the barley chromosomes in the wheat genome was confirmed with simple sequence repeat markers. The morphological characterization of the new addition lines is also discussed. Studies of the genetic stability of the whole set (2H, 3H, 4H, 7H, 1HS iso, 6HS) of 'Martonvasari 9 kr1' - 'Igri' additions revealed that the most stable disomic additions are 2H and 3H and the most unstable line is the 1HS isochromosomic addition.