Journal
JOURNAL OF VIROLOGY
Volume 89, Issue 9, Pages 5176-5179Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00339-15
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Funding
- United Kingdom charity Pancreatic Cancer Research Fund
- Ministry of Sciences and Technology, China [2013DFG32080]
- Cancer Research UK [12008] Funding Source: researchfish
- Pancreatic Cancer UK [2010 Grant - Wang] Funding Source: researchfish
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Vaccinia virus (VACV) continues to be used in immunotherapy for the prevention of infectious diseases and treatment of cancer since its use for the eradication of smallpox. However, the current method of editing the VACV genome is not efficient. Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome rapidly and efficiently. Additionally, a set of 8,964 computationally designed unique guide RNAs (gRNAs) targeting all VACV genes will be valuable for the study of VACV gene functions.
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