Journal
GENETICS AND MOLECULAR RESEARCH
Volume 13, Issue 3, Pages 5025-5035Publisher
FUNPEC-EDITORA
DOI: 10.4238/2014.July.4.18
Keywords
Non-heading Chinese cabbage; Pollen germination; Hierarchical clustering; Self-incompatibility; Expression patterns
Funding
- National Basic Research Program of China (973 Program) [2009CB119001]
- Fundamental Research Funds for the Central Universities of China [KYZ201111]
- Natural Science Foundation of Jiangsu Province [BK2011643]
- Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [BO201300666]
- Science and Technology Pillar Program of Jiangsu Province [BE2012325]
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In Brassicaceae, a self-incompatibility (SI) system mediates pollen-pistil interactions. Self-pollen could be recognized and rejected by incompatible pistils. Several components involved in the SI response have been determined, including S-locus receptor kinase (SRK), S-locus cysteine-rich protein/S-locus protein 11, and arm repeat-containing protein 1 (ARC1). However, the components involved in the SI system of Brassicaceae are not fully understood. Here, we detected expression patterns of 24 SI-related genes in non-heading Chinese cabbage (Brassica campestris ssp chinensis Makino) after compatible and incompatible pollination, and potential interaction relationships of these genes were predicted. SRK and ARC1 transcripts increased initially 0.25 h after incompatible pollination, while kinase-associated protein phosphatase had an expression pattern that was opposite that of SRK transcripts during self-pollination. Plant U-box 8 was not required in the SI response of non-heading Chinese cabbage. Our results showed that the SI signal of non-heading Chinese cabbage could occur within 0.25 h after self-pollination. The hypothetical interaction relationships indicated that plastid-lipid-associated protein and malate dehydrogenase could be negatively regulated by chaperonin 10, glutathione transferase, cytidylate kinase/uridylate kinase, and methionine synthase by indirect interactions. Our findings could be helpful to better understand potential roles of these components in the SI system of non-heading Chinese cabbage.
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