Journal
JOURNAL OF VIROLOGY
Volume 89, Issue 15, Pages 8077-8081Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00356-15
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Funding
- Natural Science Foundation of China [31272572]
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The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of the putative fusion peptide (PEDV-SFCS). PEDV-SFCS exhibited trypsin-independent cell-cell fusion and was able to replicate in culture cells independently of trypsin, though to low titer.
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