Journal
GENETICS AND MOLECULAR RESEARCH
Volume 12, Issue 4, Pages 4213-4221Publisher
FUNPEC-EDITORA
DOI: 10.4238/2013.October.7.7
Keywords
Wheat; MicroRNA; Northern blot; UV-B radiation
Funding
- National Key Basic Research Program of China [2013CB127700]
- Nature Science Foundation of China [31071651]
- 111 Project from the Ministry of Education of China [B07049]
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MicroRNAs (miRNAs) play critical roles in post-transcriptional gene regulation and act as important endogenous regulators to various stressors. Ultraviolet-B (UV-B) radiation is a major factor influencing crop growth and development. In this study, we isolated a novel wheat miRNA, named Tae-miR6000, and confirmed its expression diversity after UV-B treatments. Additionally, using the Northern blotting technique, we found that six miRNAs were highly responsive to UV-B stress in wheat. Of these six miRNAs, miR159, miR167a, and miR171 were significantly upregulated, and the remaining three miRNAs were downregulated, at different time points after UV-B treatment. This result indicates that miRNAs may be involved in the regulation of targets after induction by UV-B stress. Furthermore, promoter analysis of the UV-B-responsive miRNA genes revealed some light-relevant cis-elements, such as the I-box and G-box. Taken together, the results of this study suggest that wheat miRNAs play important roles in the response to UV-B stress.
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