Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 218, Issue -, Pages 19-22Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2015.03.004
Keywords
West Nile virus; American crow; Peripheral blood mononuclear cells; Leukocytes; Avian
Funding
- U.S. Centers for Disease Control and Prevention
- American Society for Microbiology/CDC postdoctoral fellowship
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West Nile virus (WNV) replicates in a wide variety of avian species, which act as amplification hosts. In particular, WNV generates high titers and elicits severe pathology in American crows (AMCRs; Corvus brachyrhynchos), a species that has been used as a sentinel for WNV transmission. Although the specific cellular targets of WNV replication in AMCRs are not well defined, preliminary evidence suggests that leukocytes may be an important target of early replication. Therefore, development of a protocol for ex vivo culture of AMCR leukocytes as a model for assessing differential avian host susceptibility is described herein. WNV growth in these cultures mirrored in vivo viremia profiles. These data indicate that ex vivo leukocyte cultures can be used for preliminary pathological assessment of novel WNV strains and potentially of other flaviviruses that use avian reservoir hosts. Published by Elsevier B.V.
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