Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 214, Issue -, Pages 37-42Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2015.01.005
Keywords
HIV; Early infant diagnosis; Provirus; Polymerase chain reaction
Funding
- Bill and Melinda Gates Foundation Grand Challenges in Global Health [37774]
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Early diagnosis and access to treatment for infants with human immunodeficiency virus-1 (HIV-1) is critical to reduce infant mortality. The lack of simple point-of-care tests impedes the timely initiation of antiretroviral therapy. The development of FINA, filtration isolation of nucleic acids, a novel DNA extraction method that can be performed by clinic personnel in less than 2 min has been reported previously. In this report, significant improvements in the DNA extraction and amplification methods are detailed that allow sensitive quantitation of as little as 10 copies of HIV-1 proviral DNA and detection of 3 copies extracted from 100 mu l of whole blood. An internal control to detect PCR inhibition was also incorporated. In a preliminary field evaluation of 61 South African infants, the FINA test demonstrated 100% sensitivity and specificity. The proviral copy number of the infant specimens was quantified, and it was established that 100 microliters of whole blood is required for sensitive diagnosis of infants. (C) 2015 The Authors. Published by Elsevier B.V.
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