Journal
GENETICS AND MOLECULAR RESEARCH
Volume 11, Issue 3, Pages 2855-2861Publisher
FUNPEC-EDITORA
DOI: 10.4238/2012.August.27.1
Keywords
Genetic diversity; Achillea species; ISSR; RAPD; Marker
Funding
- Iran National Science Foundation (INSF)
- Forests, Range and Watershed Management Organization of Iran
- University of Tehran
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Cultivation and preservation of yarrow has recently attracted wide attention due to its beneficial properties; however, genetic variation of Achillea species is still relatively unknown. We used RAPD and ISSR markers to assess genetic diversity in 16 accessions of yarrow belonging to two species native to Iran. Seven ISSR and nine RAPD primers generated 187 amplified fragments, of which 159 were polymorphic. The similarity coefficient among Achillea tenuifolia accessions ranged from 61 to 86%, and from 40 to 84% among A. santolina accessions. A low similarity was observed between these two species (mean similarity = 0.36%). This low similarity is consistent with their geographical distribution. According to the results of cluster and PCA analyses, the two species completely separated from each other. These markers will aid in the identification of elite genotypes for domestication and breeding programs.
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