Journal
GENETICS AND MOLECULAR RESEARCH
Volume 10, Issue 4, Pages 3559-3564Publisher
FUNPEC-EDITORA
DOI: 10.4238/2011.October.31.3
Keywords
Mini-barcode; Cytochrome oxidase 1; Reptiles; Mammals; Birds; PCR; Barcoding; Amplification success
Funding
- King Saud University [RGP-VPP-009]
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In recent years, DNA barcoding has emerged as a powerful tool for species identification. We report an extended validation of a universal DNA mini-barcode for amplification of 130-bp COI segments from 23 specimens collected from a desert environment, including 11 reptiles, five mammals and seven birds. Besides the standard double-annealing protocol, we also tested a more stringent single-annealing protocol. The PCR success rate for the amplification of the mini-barcode region was: mammals (4/5), reptiles (5/11) and birds (4/7). These findings demonstrate the limited utility of universal primers for mini-barcoding, at least for these vertebrate taxa that we collected from the Saudi Arabian desert.
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