Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 225, Issue -, Pages 64-69Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2015.09.003
Keywords
Newcastle disease virus; Phosphoprotein; Pathogenicity; Negative sense RNA; Prokaryotic expression
Funding
- Department of Biotechnology [NER-BPMC/2013/134/AAB21]
- Department of Science and Technology [IFA-LSBM-34]
- Board of Research on Nuclear Sciences [2012/20/37B/06/BRNS]
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Newcastle disease virus (NDV) is the causative agent of a highly contagious disease in avian species. All strains of NDV belong to avian paramyxovirus serotype-1. The disease is endemic in different parts of the world and vaccination is the only way to protect birds from NDV infection. The virus non-structural phosphoprotein (P) is the second most abundant protein and a major modulator of viral replication. Although P protein shows lesser evolutionary divergence among NDV isolates, it is known to be highly divergent among different avian paramyxovirus serotypes. In the present study, a recombinant P protein based single serum dilution ELISA was developed which showed better sensitivity, specificity and accuracy as compared to conventional methods for NDV detection. The recombinant P protein based ELISA could be an alternative to existing diagnostics against NDV infection in chickens. (C) 2015 Elsevier B.V. All rights reserved.
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