Journal
GENETICS AND MOLECULAR BIOLOGY
Volume 32, Issue 4, Pages 864-867Publisher
SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572009005000076
Keywords
microturbellaria; sequencing; sibling species; phylogeography; phylogeny
Funding
- Italian Ministry of Research
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Monocelis lineata lineata consists of a complex of sibling species, widespread in the Mediterranean and Atlantic Ocean. Previous genetic analysis placed in evidence at least four sibling species. Nevertheless, this research was not conclusive enough to fully resolve the complex or to infer the phylogeny/phylogeography of the group. We designed specific primers aiming at obtaining partial sequences of the mtDNA gene Cytochrome c Oxidase subunit I (COI) of M. lineata, and have identified 25 different haplotypes in 32 analyzed individuals. The dendrogram generated by Neighbor-Joining analysis confirmed the differentiation between Atlantic and Mediterranean siblings, as well as the occurrence of at least two Mediterranean sibling species. Thus validated, the method here presented appears as a valuable tool in population genetics and biodiversity surveys on the Monocelis lineata complex.
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