Journal
GENETICS AND MOLECULAR BIOLOGY
Volume 31, Issue 1, Pages 166-171Publisher
SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572008000100028
Keywords
Brachyteles; conservation genetics; fecal DNA; mtDNA; PCR-RFLP
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We assessed the genetic diversity of two northern muriqui (Brachyteles hypoxanthus Primata, Ateliclae) populations, the Feliciano Miguel Abdala population (FMA, n = 108) in the Brazilian state of Minas Gerais (19 degrees 44'S, 41 degrees 49'W) and the Santa Maria de Jetiba population (SMJ, n = 18) in the Brazilian state of Espirito Santo (20 degrees 01'S, 40 degrees 44'W). Fecal DNA was isolated and PCR-RFLP analysis used to analyze 2160 bp of mitochondrial DNA, made up of an 820 bp segment of the gene cytochrome c oxidase subunit 2 (cox2, EC 1.9.3.1), an 880 bp segment of the gene cytochrome b (cytb, EC 1. 10.2.2) and 460 bp of the hypervariable segment of the mtDNA control region (HVRI). The cox2 and cytb sequences were monomorphic within and between populations whereas the HVRI revealed three different population exclusive haplotypes, one unique to the SMJ population and two, present at similar frequencies, in the FMA population. Overall haplotype diversity (h = 0.609) and nucleoticle diversity (pi = 0.181) were high but reduced within populations. The populations were genetically structured with a high fixation index (F-ST = 0.725), possibly due to historical subdivision. These findings have conservation implications because they seem to indicate that the populations are distinct management units.
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