Journal
GENETICS AND MOLECULAR BIOLOGY
Volume 31, Issue 3, Pages 717-724Publisher
SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572008000400019
Keywords
chromosome markers; DAPI bands; rDNA loci; dot blot hybridization; genome relationships
Funding
- European Commission
- INCO-DEV [ICA4-CT-2001-10072]
- Consultative Group on International Agricultural Research
- onsenjo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina
- ANPCyT, Argentina [PICT 2005 n. 34458]
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Chromosome markers were developed for Arachis glandulifera using fluorescence in situ hybridization (FISH) of the 5S and 45S rRNA genes and heterochromatic 4'-6-diamidino-2-phenylindole (DAPI) positive bands. We used chromosome landmarks identified by these markers to construct the first Arachis species ideogram in which all the homologous chromosomes were precisely identified. The comparison of this ideogram with those published for other Arachis species revealed very poor homeologies with all A and B genome taxa, supporting the special genome constitution (D genome) of A. glandulifera. Genomic affinities were further investigated by dot blot hybridization of biotinylated A. glandulifera total DNA to DNA from several Arachis species, the results indicating that the D genome is positioned between the A and B genomes.
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