Journal
GENETICS
Volume 192, Issue 1, Pages 55-66Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.112.140590
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Funding
- American Heart Association [10SDG4310061]
- National Institute of General Medical Sciences/National Institutes of Health (NIH) [R01GM096032]
- National Heart, Lung, and Blood Institute/NIH [R01HL108643]
- New York Cardiac Center
- New York University College of Arts and Sciences
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The experimental malleability and unique phylogenetic position of the sea squirt Ciona intestinalis as part of the sister group to the vertebrates have helped establish these marine chordates as model organisms for the study of developmental genetics and evolution. Here we summarize the tools, techniques, and resources available to the Ciona geneticist, citing examples of studies that employed such strategies in the elucidation of gene function in Ciona. Genetic screens, germline transgenesis, electroporation of plasmid DNA, and microinjection of morpholinos are all routinely employed, and in the near future we expect these to be complemented by targeted mutagenesis, homologous recombination, and RNAi. The genomic resources available will continue to support the design and interpretation of genetic experiments and allow for increasingly sophisticated approaches on a high-throughput, whole-genome scale.
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