Journal
GENETICS
Volume 191, Issue 4, Pages 1381-U476Publisher
GENETICS SOC AM
DOI: 10.1534/genetics.112.141341
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Funding
- European Research Council
- Swiss National Science Foundation
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We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds.
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