A Genetic Test for Yeast Two-Hybrid Bait Competency Using RanBPM

We describe a simple genetic test for assessing the competency of Gal4-based baits prior to a yeast two-hybrid screen, which allows determination of whether a bait protein is expressed appropriately for an interaction to be detected. The novel test, based on interaction with the protein RanBPM, is easier and More predictive than offer methods such as Western blotting, allowing identification of similar to 80% of incompetent baits prior to screening.