Journal
GENETICS
Volume 183, Issue 4, Pages 1397-1419Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.109.110171
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Funding
- NIGMS NIH HHS [GM084673, GM34059, R01 GM034059, F32 GM084673] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [F32GM084673, R01GM034059] Funding Source: NIH RePORTER
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P granules are non-membrane-bound organelles found in the germ-line cytoplasm throughput Caenorhabditis elegans development. Like their germ granule counterparts in other animals, P granules are thought to act as determinants of the identity and special properties of germ cells, properties that include tire unique ability to give rise to all tissues of future generations of an organism. Therefore, understanding how P granules work is critical to understanding how cellular immortality and totipotency are retained, gained, and lost. Here we report on a genome wide RNAi screen in C. elegans, which identified 173 genes that affect the stability, localization, and function of P granules. Many of these genes fall into specific classes with shared P-granule phenotypes, allowing Lis to better understand how cellular processes such as protein degradation, translation, splicing, nuclear transport, and mRNA homeostasis converge on P-granule assembly and function. One of the more striking phenotypes is caused by the depletion of CSR-1, an Argonaute associated with an endogenous siRNA pathway that functions in the germ line. We show that CSR-1 and two other endo-siRNA pathway members, the RNA-dependent, RNA polymerase EGO-1 and the helicase DRH-3, act to antagonize RNA and P-granule accumulation in the germ line. our findings Strengthen the emerging view that germ grannies are involved in numerous aspects of RNA metabolism, including an endo-siRNA pathway in germ cells.
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