Journal
GENETICS
Volume 178, Issue 2, Pages 711-723Publisher
GENETICS
DOI: 10.1534/genetics.107.081331
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Funding
- NCI NIH HHS [R13 CA162528] Funding Source: Medline
- NIGMS NIH HHS [R01 GM020056-33, R01 GM059413-07, GM59413, R01 GM061766-06, R01 GM059413, R01 GM061766-07, GM56888, R37 GM059413, R01 GM061766, R01 GM020056, R01 GM061766-08, R01 GM056888, GM61766] Funding Source: Medline
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The Mre11 complex functions in double-strand break (DSB) repair, meiotic recombination, and DNA damage checkpoint pathways. Sae2 deficiency has opposing effects on the Mre11 complex. On one hand, it appears to impair Mre11 nuclease function in DNA repair and meiotic DSB processing, and on the other, Sae2 deficiency activates Mre11-complex-dependent DNA-damage-signaling via the Te11-Mre11 complex (TM) pathway. We demonstrate that SAE2 overexpression blocks the TM pathway, suggesting that Sae2 antagonizes Mre11 1-complex checkpoint functions. To understand how Sae2 regulates the Mre11 complex, we screened for sae2 alleles that behaved as the null with respect to Mre11-complex checkpoint functions, but left nuclease function intact. Phenotypic characterization of these sae2 alleles suggests that Sae2 functions as a multimer and influences the substrate specificity of the Mre11 nuclease. We show that Sae2 oligomerizes independently of DNA damage and that oligomerization is required for its regulatory influence on the Mre11 nuclease and checkpoint functions.
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