Journal
GENETICS
Volume 180, Issue 1, Pages 703-707Publisher
GENETICS
DOI: 10.1534/genetics.108.090563
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Funding
- Howard Hughes Medical Institute Funding Source: Medline
- NIMH NIH HHS [R01 MH084234] Funding Source: Medline
- NINDS NIH HHS [R01 NS040929, R01 NS040929-08, R37 NS040929] Funding Source: Medline
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In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-positive targeting candidates.
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