Journal
JOURNAL OF VETERINARY MEDICAL SCIENCE
Volume 77, Issue 11, Pages 1527-1529Publisher
JAPAN SOC VET SCI
DOI: 10.1292/jvms.14-0367
Keywords
ampicillin; LC-MS/MS; medicated feed; residue level; swine tissue
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We assessed ampicillin (ABPC) concentrations of kidney, muscle and intestine after a 5 day withdrawal period in 2 male and a female young Large White pigs fed the diet containing ABPC (ABPC medicated feed, 24 mg/kg/day) for a week. The ABPC residues were measured with liquid chromatography tandem mass spectrometry, and the mean recoveries and quantitation limits ranged from 91.8 to 97.2% and from 0.1 to 0.12 ng/g, respectively. The residual ABPC concentrations were <= 1.18 ng/g for the muscle, <= 0.53 ng/g for the kidney and <= 1.93 ng/g for the intestine, suggesting below the Japanese provisional maximum residue limits. These results reveal that the analytical method is developed for residual ABPC and that the withdrawal period is appropriate.
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