Journal
GENESIS
Volume 50, Issue 12, Pages 914-920Publisher
WILEY
DOI: 10.1002/dvg.22341
Keywords
Drosophila; GAL4-UAS; testis; spermatogonia; spermatocytes
Categories
Funding
- Australian Research Council Centre of Excellence in Biotechnology and Development
- Discovery Project [120100224]
Ask authors/readers for more resources
The Drosophila testis has proven to be a valuable model organ for investigation of germline stem cell (GSC) maintenance and differentiation as well as elucidation of the genetic programs that regulate differentiation of daughter spermatogonia. Development of germ cell specific GAL4 driver transgenes has facilitated investigation of gene function in GSCs and spermatogonia but specific GAL4 tools are not available for analysis of postmitotic spermatogonial differentiation into spermatocytes. We have screened publically available pGT1 strains, a GAL4-encoding gene trap collection, to identify lines that can drive gene expression in late spermatogonia and early spermatocytes. While we were unable to identify any germline-specific drivers, we did identify an insertion in the chiffon locus, which drove expression specifically in early spermatocytes within the germline along with the somatic cyst cells of the testis. genesis 50: 914-920, 2012. (C) 2012 Wiley Periodicals, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available