Journal
GENESIS
Volume 48, Issue 10, Pages 603-606Publisher
WILEY
DOI: 10.1002/dvg.20659
Keywords
mouse; mutagenesis; gene trap; FLP; site-specific recombinase; ES cells
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Funding
- National Institutes of Child Health and Human Development [RO1HD24875, R37HD25326]
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Gene trap mutagenesis in embryonic stem cells is an important tool to help elucidate gene function in current mouse mutagenesis efforts. Vector systems based on inversion of the gene trap module have recently been devised to allow for conditional mutagenesis. However, additional efforts are needed to improve this technology including improving the efficiency of site-specific recombinases required to manipulate these conditional vectors in vivo. Here we describe a mouse line carrying the codon-optimized FIR recombinase Flpo at the ROSA26 locus that functions at higher efficiency than a similar Flpe line in mediating the DNA inversion of a conditional gene trap cassette in vivo. genesis 48:603-606, 2010. (C) 2010 Wiley-Liss, Inc.
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