Journal
GENESIS
Volume 48, Issue 9, Pages 563-567Publisher
WILEY-BLACKWELL
DOI: 10.1002/dvg.20654
Keywords
Tie2; Cre recombinase; hematopoietic lineage; mouse transgenic; ROSA reporter
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Funding
- NIH [R01 HL070865, HL65301, P20RR1555, P20RR181789]
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The regulatory elements of the Tie2/Tek promoter are commonly used in mouse models to direct transgene expression to endothelial cells. Tunica intima endothelial kinase 2 (Tie2) is also expressed in hematopoietic cells, although this has not been fully characterized. We determine the lineages of adult hematopoietic cells derived from Tie2-expressing populations using Tie2-Cre;Rosa26R-EYFP mice. In Tie2-Cre;Rosa26-REYFP mice, analysis of bone marrow cells showed Cre-mediated recombination in 85% of the population. In adult bone marrow and spleen, we analyzed subclasses of early hematopoietic progenitors, T cells, monocytes, granulocytes, and B cells. We found that similar to 84% of each lineage was EYFP+, and nearly all cells that come from Tie2-expressing lineages are CD45(+), confirming widespread contribution to definitive hematopoietic cells. In addition, more than 82% of blood cells within the embryonic yolk sac were of Tie2(+) origin. Our findings of high levels of Tie2-Cre recombination in the hematopoietic lineage have implications for the use of the Tie2-Cre mouse as a lineage-restricted driver strain. genesis 48:563-567, 2010. (C) 2010 Wiley-Liss, Inc.
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