Journal
GENESIS
Volume 46, Issue 12, Pages 711-718Publisher
WILEY
DOI: 10.1002/dvg.20435
Keywords
lentiviral vector; transgenesis; integration; preference genome; mouse zygote
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Funding
- NIH (Yerkes National Primate Research Center) [RR-00165]
- NCRR/NIH [RR018827-04]
- NATIONAL CENTER FOR RESEARCH RESOURCES [P51RR000165, R24RR018827] Funding Source: NIH RePORTER
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Lentiviral gene transfer has a significant impact on the development of biomedical research. One of the most important features of lentiviruses is the capability to infect both dividing and nondividing cells. However, little is known whether integration preference exists, specifically in early embryos. An in-depth genome analysis on 112 independent lentiviral integration sites from 43 transgenic founder mice was performed to determine if there are preferable sites for lentiviral integration in early embryonic genome. Our results demonstrated that lentiviruses were biased in integrating within intragenic regions, especially in the introns. However, no integration preference was found associated with specific chromosomes, repetitive elements, or CpG islands, nor was there any preference for integrating at close proximity to transcription start sites. Our findings suggested that lentiviruses were biased to integrate into the intragenic regions of early embryonic genome of mouse. genesis 46:711-718, 2008. (C) 2008 Wiley-Liss, Inc.
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