Journal
GENES TO CELLS
Volume 20, Issue 3, Pages 173-190Publisher
WILEY
DOI: 10.1111/gtc.12211
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT)
- Global Center of Excellence grant from MEXT
- Japan Society for the Promotion of Science for Young Scientists
- Grants-in-Aid for Scientific Research [26440187, 25000013] Funding Source: KAKEN
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The molecular machinery of the cyanobacterial circadian clock oscillator consists of three proteins, KaiA, KaiB and KaiC, which interact with each other to generate circadian oscillations in the presence of ATP (the in vitro KaiABC clock oscillator). KaiB comprises four subunits organized as a dimer of dimers. Our previous study suggested that, on interaction with KaiC, the tetrameric KaiB molecule dissociates into two molecules of dimeric KaiB. It is uncertain whether KaiB also exists as a monomer and whether the KaiB monomer can drive normal circadian oscillation. To address these questions, we constructed a new KaiB oligomer mutant with an N-terminal deletion, KaiB(10-108). KaiB(10-108) was a monomer at 4 degrees C but a dimer at 35 degrees C. KaiB(10-108) was able to drive normal clock oscillation in an in vitro reconstituted KaiABC clock oscillator at 25 degrees C, but it was not able to drive normal circadian gene expression rhythms in cyanobacterial cells at 41 degrees C. Wild-type KaiB existed in equilibrium between a dimer and tetramer at lower KaiB concentrations or in the presence of 1m NaCl. Our findings suggest that KaiB is in equilibrium between a monomer, dimer and tetramer in cyanobacterial cells.
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