Journal
GENES TO CELLS
Volume 19, Issue 6, Pages 528-538Publisher
WILEY-BLACKWELL
DOI: 10.1111/gtc.12149
Keywords
-
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) KAKENHI [23112716, 24770168]
- Japan Science and Technology Agency (CREST)
- Dai-ichi Sankyo TaNeDS
- Astellas Foundation for Research on Metabolic Disorders
- Grants-in-Aid for Scientific Research [26650062, 24770168] Funding Source: KAKEN
Ask authors/readers for more resources
Human centromeres consist of repetitive sequences from which satellite I noncoding RNAs are transcribed. We found that knockdown of satellite I RNA causes abnormal chromosome segregation and generation of nuclei with a grape-shape phenotype. Co-immunoprecipitation experiments showed that satellite I RNA associates with Aurora B, a component of the chromosome passenger complex (CPC) regulating proper attachment of microtubules to kinetochores, in mitotic HeLa cells. Satellite I RNA was also shown to associate with INCENP, another component of the CPC. In addition, depletion of satellite I RNA resulted in up-regulation of kinase activity of Aurora B and delocalization of the CPC from the centromere region. These results suggest that satellite I RNA is involved in chromosome segregation through controlling activity and centromeric localization of Aurora B kinase.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available