Journal
GENES & GENOMICS
Volume 33, Issue 3, Pages 245-250Publisher
SPRINGER
DOI: 10.1007/s13258-010-0114-6
Keywords
Jeju (Cheju) native horse; Normalization; Real-time RT-PCR; Reference genes; Thoroughbred
Categories
Funding
- Agricultural R&D Promotion Center (ARPC) [109019-03-1-SB020]
Ask authors/readers for more resources
Quantitative analysis of horse gene expression profiles under diverse experimental conditions is limited by the lack of reliable reference genes for normalization of mRNA levels. Therefore, in this study, the expression of potential reference genes was compared between thoroughbred and Jeju native horse (Jeju pony). We compared the expression of nine genes by quantitative real-time RT-PCR in fourteen tissues between the two horse breeds and analyzed their stability using the geNorm and NormFinder programs. The data obtained in this study suggest that the UBB gene could serve as a reference gene in gene expression analysis of thoroughbred and Jeju native horses.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available