Journal
GENES & GENETIC SYSTEMS
Volume 87, Issue 2, Pages 137-143Publisher
GENETICS SOC JAPAN
DOI: 10.1266/ggs.87.137
Keywords
allopolyploidization; chromosomal synteny; high resolution melting analysis; single nucleotide polymorphism; synthetic hexaploid wheat
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [21380005, 23658010]
- Sumitomo Foundation
- Grants-in-Aid for Scientific Research [21380005, 23658010] Funding Source: KAKEN
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Available information on genetically assigned molecular markers is not sufficient for efficient construction of a high-density linkage map in wheat. Here, we report on application of high resolution melting (HRM) analysis using a real-time PCR apparatus to develop single nucleotide polymorphism (SNP) markers linked to a hybrid necrosis gene, Net2, located on wheat chromosome 2D. Based on genomic information on barley chromosome 2H and wheat expressed sequence tag libraries, we selected wheat cDNA sequences presumed to be located near the Net2 chromosomal region, and then found SNPs between the parental Ae. tauschii accessions of the synthetic wheat mapping population. HRM analysis of the PCR products from F2 individuals' DNA enabled us to assign 44.4% of the SNP-representing cDNAs to chromosome 2D despite the presence of the A and B genomes. In addition, the designed SNP markers were assigned to chromosome 2D of Ae. tauschii. The order of the assigned SNP markers in synthetic hexaploid wheat was confirmed by comparison with the markers in barley and Ae. tauschii. Thus, the SNP-genotyping method based on HRM analysis is a useful tool for development of molecular markers at target loci in wheat.
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