Journal
GENES & DEVELOPMENT
Volume 28, Issue 11, Pages 1239-1251Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.240192.114
Keywords
CsrA; Rho factor; transcription attenuation; bacterial gene regulation
Categories
Funding
- CNRS
- French Agence Nationale de la Recherche [ANR-13-BSV3-0005-01, ANR-13-BSV3-0005-02]
- Agence Nationale de la Recherche (ANR) [ANR-13-BSV3-0005] Funding Source: Agence Nationale de la Recherche (ANR)
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RNA-binding protein CsrA is a key regulator of a variety of cellular processes in bacteria, including carbon and stationary phase metabolism, biofilm formation, quorum sensing, and virulence gene expression in pathogens. CsrA binds to bipartite sequence elements at or near the ribosome loading site in messenger RNA (mRNA), most often inhibiting translation initiation. Here we describe an alternative novel mechanism through which CsrA achieves negative regulation. We show that CsrA binding to the upstream portion of the 5' untranslated region of Escherichia call pgaA mRNA-encoding a polysaccharide adhesin export protein-unfolds a secondary structure that sequesters an entry site for transcription termination factor Rho, resulting in the premature stop of transcription. These findings establish a new paradigm for bacterial gene regulation in which remodeling of the nascent transcript by a regulatory protein promotes Rho-dependent transcription attenuation.
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