Journal
GENES & DEVELOPMENT
Volume 28, Issue 1, Pages 14-19Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.231886.113
Keywords
RNA polymerase II; promoter-proximal pausing; termination; escape to productive elongation; photoactivation; nascent RNA
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Funding
- National Institute of Health (NIH) [GM25232, GM087003]
- Howard Hughes Medical Institute
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The kinetics with which promoter-proximal paused RNA polymerase II (Pol II) undergoes premature termination versus productive elongation is central to understanding underlying mechanisms of metazoan transcription regulation. To assess the fate of Pol II quantitatively, we tracked photoactivatable GFP-tagged Pol II at uninduced Hsp70 on polytene chromosomes and showed that Pol II is stably paused with a half-life of 5 min. Biochemical analysis of short nascent RNA from Hsp70 reveals that this half-life is determined by two comparable rates of productive elongation and premature termination of paused Pol II. Importantly, heat shock dramatically increases elongating Pol II without decreasing termination, indicating that regulation acts at the step of paused Pol II entry to productive elongation.
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