Journal
GENES & DEVELOPMENT
Volume 28, Issue 14, Pages 1556-1561Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.244673.114
Keywords
tRNA splicing; tRNA ligation; intron turnover; Xrn1; Rlg1; 5 ' to 3 ' exonuclease
Categories
Funding
- National Institutes of Health [GM27930]
- The Ohio State University Pelotonia Graduate Fellowship
Ask authors/readers for more resources
In eukaryotes and archaea, tRNA splicing generates free intron molecules. Although similar to 600,000 introns are produced per generation in yeast, they are barely detectable in cells, indicating efficient turnover of introns. Through a genome-wide search for genes involved in tRNA biology in yeast, we uncovered the mechanism for intron turnover. This process requires healing of the 59 termini of linear introns by the tRNA ligase Rlg1 and destruction by the cytoplasmic tRNA quality control 5 '-to-3 ' exonuclease Xrn1, which has specificity for RNAs with 59 monophosphate.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available