Journal
GENES & DEVELOPMENT
Volume 27, Issue 7, Pages 767-777Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.216200.113
Keywords
transcription factory; RNA polymerase II; live imaging
Categories
Funding
- EuTRACC (European Transcriptome, Regulome, and Cellular Commitment Consortium)
- SyBoSS (Systems Biology of Stem Cells and Reprogramming) consortium (EU)
- Dutch Academy of Sciences (KNAW [Koninklijke Nederlandse Akademie van Wetenschappen])
- NGI (Netherlands Genomics Initiative [CBG {Centraal Bureau voor Genealogie}, Netherlands])
- Deutsche Forschungsgemeinschaft Sonderforschungsbereiche/Transregio5
Ask authors/readers for more resources
Transcription steps are marked by different modifications of the C-terminal domain of RNA polymerase II (RNAPII). Phosphorylation of Ser5 and Ser7 by cyclin-dependent kinase 7 (CDK7) as part of TFIIH marks initiation, whereas phosphorylation of Ser2 by CDK9 marks elongation. These processes are thought to take place in localized transcription foci in the nucleus, known as transcription factories,'' but it has been argued that the observed clusters/foci are mere fixation or labeling artifacts. We show that transcription factories exist in living cells as distinct foci by live-imaging fluorescently labeled CDK9, a kinase known to associate with active RNAPII. These foci were observed in different cell types derived from CDK9-mCherry knock-in mice. We show that these foci are very stable while highly dynamic in exchanging CDK9. Chromatin immunoprecipitation (ChIP) coupled with deep sequencing (ChIP-seq) data show that the genome-wide binding sites of CDK9 and initiating RNAPII overlap on transcribed genes. Immunostaining shows that CDK9-mCherry foci colocalize with RNAPII-Ser5P, much less with RNAPII-Ser2P, and not with CDK12 (a kinase reported to be involved in the Ser2 phosphorylation) or with splicing factor SC35. In conclusion, transcription factories exist in living cells, and initiation and elongation of transcripts takes place in different nuclear compartments.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available