Journal
GENES & DEVELOPMENT
Volume 27, Issue 11, Pages 1288-1298Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.220467.113
Keywords
histone code; UHRF1; DNMT1; multivalency; epigenetic inheritance; DNA methylation
Categories
Funding
- National Institutes of Health (NIH) [GM068088]
- Carolina Partnership [GM090732, GM100919]
- University Cancer Research Fund
- University of North Carolina at Chapel Hill
- Natural Sciences and Engineering Research Council of Canada [372475-10]
- University of North Carolina Line-berger Comprehensive Cancer Center Basic Sciences Training Program [T32CA09156]
- American Cancer Society [PF-13-085-01-DMC]
- Boehringer Ingelheim
- Canada Foundation for Innovation
- Canadian Institutes of Health Research
- Genome Canada through the Ontario Genomics Institute [OGI-055]
- GlaxoSmithKline
- Janssen
- Lilly Canada
- Novartis Research Foundation
- Ontario Ministry of Economic Development and Innovation
- Pfizer
- Takeda
- Wellcome Trust [092809/Z/10/Z]
Ask authors/readers for more resources
Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. While significant progress has been made characterizing individual effector domains, the role of paired domains and how they function in a combinatorial fashion within chromatin are poorly defined. Here we show that the linked tandem Tudor and plant homeodomain (PHD) of UHRF1 (ubiquitin-like PHD and RING finger domain-containing protein 1) operates as a functional unit in cells, providing a defined combinatorial readout of a heterochromatin signature within a single histone H3 tail that is essential for UHRF1-directed epigenetic inheritance of DNA methylation. These findings provide critical support for the histone code'' hypothesis, demonstrating that multivalent histone engagement plays a key role in driving a fundamental downstream biological event in chromatin.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available