Journal
GENES & DEVELOPMENT
Volume 27, Issue 13, Pages 1526-1536Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.221374.113
Keywords
circadian gene expression; real-time bioluminescence monitoring; liver oscillators; resetting cues; SCN lesion
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Funding
- Swiss National Science Foundation [SNF 31-113565, SNF 31-128656/1, SNF 31003A-130825]
- European Research Council (ERC-AdG-TimeSignal)
- Louis Jeantet Foundation of Medicine
- Canton of Geneva
- Novartis Foundation
- EUMODIC (European FP6 program) [037188]
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The mammalian circadian timing system consists of amaster pacemaker in the suprachiasmatic nucleus (SCN) in the hypothalamus, which is thought to set the phase of slave oscillators in virtually all body cells. However, due to the lack of appropriate in vivo recording technologies, it has been difficult to study how the SCN synchronizes oscillators in peripheral tissues. Here we describe the real-time recording of bioluminescence emitted by hepatocytes expressing circadian luciferase reporter genes in freely movingmice. The technology employs a device dubbed RT-Biolumicorder, which consists of a cylindrical cage with reflecting conical walls that channel photons toward a photomultiplier tube. The monitoring of circadian liver gene expression revealed that hepatocyte oscillators of SCN-lesioned mice synchronized more rapidly to feeding cycles than hepatocyte clocks of intact mice. Hence, the SCN uses signaling pathways that counteract those of feeding rhythms when their phase is in conflict with its own phase.
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