Journal
GENES & DEVELOPMENT
Volume 26, Issue 8, Pages 857-871Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.184648.111
Keywords
Polycomb; Polyhomeotic; Drosophila; mitosis; kinetics; stem cell
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Funding
- Austrian Academy of Sciences
- EU
- Portuguese Foundation for Science and Technology [SFRH/BD/40389/2007]
- Austrian Science Fund [FWF P22340]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/40389/2007] Funding Source: FCT
- Austrian Science Fund (FWF) [P 22340] Funding Source: researchfish
- Austrian Science Fund (FWF) [P22340] Funding Source: Austrian Science Fund (FWF)
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Epigenetic memory mediated by Polycomb group (PcG) proteins must be maintained during cell division, but must also be flexible to allow cell fate transitions. Here we quantify dynamic chromatin-binding properties of PH::GFP and PC:: GFP in living Drosophila in two cell types that undergo defined differentiation and mitosis events. Quantitative fluorescence recovery after photobleaching (FRAP) analysis demonstrates that PcG binding has a higher plasticity in stem cells than in more determined cells and identifies a fraction of PcG proteins that binds mitotic chromatin with up to 300-fold longer residence times than in interphase. Mathematical modeling examines which parameters best distinguish stem cells from differentiated cells. We identify phosphorylation of histone H3 at Ser 28 as a potential mechanism governing the extent and rate of mitotic PC dissociation in different lineages. We propose that regulation of the kinetic properties of PcG-chromatin binding is an essential factor in the choice between stability and flexibility in the establishment of cell identities.
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