Journal
GENES & DEVELOPMENT
Volume 26, Issue 20, Pages 2271-2285Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.196451.112
Keywords
Trop2; self-renewal; transformation; regulated intramembrane proteolysis; beta-catenin
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Funding
- California Institute for Regenerative Medicine Training Grant [TG2-01169]
- Department of Defense Prostate Cancer Research Program [PC110638, PC101928, W81XWH-11-1-0227, W81XWH-12-1-0206]
- SPORE in prostate cancer
- Joe and Ali Torre-Prostate Cancer Foundation Young Investigator Award
- UCLA Tumor Biology Program (Ruth L. Kirschstein Institutional National Research Service) [T32 CA009056]
- UCLA SPORE in Prostate Cancer
- National Cancer Institute [1R01CA158627-01]
- Prostate Cancer Foundation Challenge Award
- Prostate Cancer Foundation Creativity Award
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The cell surface protein Trop2 is expressed on immature stem/progenitor-like cells and is overexpressed in many epithelial cancers. However the biological function of Trop2 in tissue maintenance and tumorigenesis remains unclear. In this study, we demonstrate that Trop2 is a regulator of self-renewal, proliferation, and transformation. Trop2 controls these processes through a mechanism of regulated intramembrane proteolysis that leads to cleavage of Trop2, creating two products: the extracellular domain and the intracellular domain. The intracellular domain of Trop2 is released from the membrane and accumulates in the nucleus. Heightened expression of the Trop2 intracellular domain promotes stem/progenitor self-renewal through signaling via beta-catenin and is sufficient to initiate precursor lesions to prostate cancer in vivo. Importantly, we demonstrate that loss of b-catenin or Trop2 loss-of-function cleavage mutants abrogates Trop2-driven self-renewal and hyperplasia in the prostate. These findings suggest that heightened expression of Trop2 is selected for in epithelial cancers to enhance the stem-like properties of self-renewal and proliferation. Defining the mechanism of Trop2 function in self-renewal and transformation is essential to identify new therapeutic strategies to block Trop2 activation in cancer.
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