Journal
GENES & DEVELOPMENT
Volume 24, Issue 21, Pages 2389-2394Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1974810
Keywords
Urp; U2AF35; splicing; spliceosome assembly; U2-type intron; U12-type intron
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Funding
- Ministry of Education, Science, and Technology (MEST) [2010-0000437]
- Gwangju Institute of Science and Technology
- Brain Korea 21 Project Research Foundation
- National Institutes of Health [R01GM035490]
- Ministry of Education, Science & Technology (MoST), Republic of Korea [gist-11] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [과06A1202] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The U2AF35-related protein Urp has been implicated previously in splicing of the major class of U2-type introns. Here we show that Urp is also required for splicing of the minor class of U12-type introns. Urp is recruited in an ATP-dependent fashion to the U12-type intron 39 splice site, where it promotes formation of spliceosomal complexes. Remarkably, Urp also contacts the 39 splice site of a U2-type intron, but in this case is specifically required for the second step of splicing. Thus, through recognition of a common splicing element, Urp facilitates distinct steps of U2- and U12-type intron splicing.
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