Journal
GENES & DEVELOPMENT
Volume 23, Issue 20, Pages 2400-2404Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1831509
Keywords
HARP; annealing helicase; RPA; SMARCAL1; SIOD
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Funding
- National Institutes of Health [GM058272, CA100710, F32 GM76936]
- Department of Defense Breast Cancer Research Program [DAMD17-03-1-0436]
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HepA-related protein (HARP) (also known as SMARCAL1) is an ATP-driven annealing helicase that catalyzes the formation of dsDNA from complementary Replication protein A (RPA)-bound ssDNA. Here we find that HARP contains a conserved N-terminal motif that is necessary and sufficient for binding to RPA. This RPA-binding motif is not required for annealing helicase activity, but is essential for the recruitment of HARP to sites of laser-induced DNA damage. These findings suggest that the interaction of HARP with RPA increases the concentration of annealing helicase activity in the vicinity of ssDNA regions to facilitate processes such as DNA repair.
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