Journal
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
Volume 27, Issue 3, Pages 287-294Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1040638715580982
Keywords
Aleutian mink disease virus; American mink; counterimmunoelectrophoresis; polymerase chain reaction
Categories
Funding
- Canada Mink Breeders Association
- Nova Scotia Mink Breeders Association
- Fur Farmers of Canada Marketing Association
- American Legend Cooperatives
- North American Fur Auctions
- American Mink Council
- American Mink Farmers Research Foundation
- Agriculture and Agri-Food Canada through the CARD Council of Ontario (Agri-Futures Nova Scotia)
- Agriculture and Agri-Food Canada through the CARD Council of British Columbia (Agri-Futures Nova Scotia)
- Agriculture and Agri-Food Canada through the CARD Council of Nova Scotia (Agri-Futures Nova Scotia)
- Atlantic Innovation Fund of Atlantic Opportunity Agency
- Technology Development Program of the Nova Scotia Department of Agriculture
Ask authors/readers for more resources
Early detection of infection by the Aleutian mink disease virus (AMDV; Carnivore amdoparvovirus 1) by polymerase chain reaction (PCR) or counterimmunoelectrophoresis (CIEP) has important ramifications in virus eradication programs. A spleen homogenate containing a local isolate of AMDV was injected intraperitoneally into black (n = 44) and sapphire (n = 12) American mink (Neovison vison). Animals were euthanized 10 days postinoculation and anti-AMDV antibodies and AMDV DNA were tested in plasma and 7 organs by CIEP and PCR, respectively. Viral DNA was detected in the plasma, spleen, lymph nodes, bone marrow, and lung samples of all inoculated mink, but was not detected in some small intestine, kidney, and liver samples. In contrast, antibodies were detected in the plasma of 3 sapphire (25.0%) and 19 black (43.2%) mink but not in any of the organs. The sensitivity of the CIEP test on plasma samples was 39.3%, implying that low levels of antibodies during the early stages of virus exposure resulted in failure to detect infection by the CIEP test. We concluded that CIEP is not a reliable test for early detection of AMDV infection in mink and that there were considerable differences among mink of each color type for production of detectable levels of antibodies. PCR tests on samples of saliva, rectal swabs, and feces did not produce consistent and reliable results.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available