Journal
GENES & DEVELOPMENT
Volume 23, Issue 12, Pages 1379-1386Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1788009
Keywords
Gene regulation; RNA-binding protein; RNA processing; RNA-Seq; transcriptome
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Funding
- Canadian Institutes of Health Research
- National Cancer Institute of Canada
- Ontario Genomics Institute
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Recent papers have described the first application of high-throughput sequencing (HTS) technologies to the characterization of transcriptomes. These studies emphasize the tremendous power of this new technology, in terms of both profiling coverage and quantitative accuracy. Initial discoveries include the detection of substantial new transcript complexity, the elucidation of binding maps and regulatory properties of RNA-binding proteins, and new insights into the links between different steps in pre-mRNA processing. We review these findings, focusing on results from profiling mammalian transcriptomes. The strengths and limitations of HTS relative to microarray profiling are discussed. We also consider how future advances in HTS technology are likely to transform our understanding of integrated cellular networks operating at the RNA level.
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