Journal
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Volume 194, Issue -, Pages 31-44Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2013.08.014
Keywords
Bisphenol A; Gobiocypris rarus; Steroidogenesis-related gene; Sex steroid hormone receptor; Gonadotropin receptor; Transcription factor
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Funding
- National Natural Science Foundation of China [31270547]
- Natural Science Foundation of Shaanxi Province, China [2011JM3009]
- Fundamental Research Funds for the Central Universities in Northwest AF University [QN2011062]
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Bisphenol A (BPA) widely used in the manufacture of numerous products is ubiquitous in aquatic environment. To explore the mechanisms of BPA-mediated actions, male rare minnow Gobiocypris rarus were exposed to BPA at concentrations of 5, 15, and 50 mu g/L for 14 and 35 days in the present study. Four subtypes of nr5a gene encoding important transcription factors for steroidogenesis were characterized, and tissue distribution analysis demonstrated distinct expression profiling of the four genes in G. rarus. BPA at environmentally relevant concentration (5 mu g/L) caused increase of gonadosomatic index (GSI) of male fish. In response to BPA, no obvious changes on the testis development were observed. Modulation of vtg mRNA expression by BPA suggests estrogenic and/or anti-estrogenic effects of BPA were dependent on exposed duration (14 or 35 days). Gene expression profiling for testicular steroidogenesis-related genes, sexual steroid receptors, gonadotropin receptors, and transcription factors indicates differential regulation was dependent on exposure duration and dose of BPA. The correlation analysis at mRNA level demonstrates that the BPA-mediated actions on testicular steroidogenesis might involve sex steroid hormone receptor signaling, gonadotropin/gonadotropin receptor pathway, and transcription factors such as nuclear receptor subfamily 5, group A (Nr5a), fork head box protein L2 (Foxl2). (C) 2013 Elsevier Inc. All rights reserved.
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