Interactive effects of hypoxia with estradiol-17β on tryptophan hydroxylase activity and serotonin levels in the Atlantic croaker hypothalamus

Hypoxia causes a marked decline in reproductive neuroendocrine function in Atlantic croaker due to decreases in the hypothalamic expression and activities of tryptophan hydroxylase (TPH, the rate limiting enzyme in serotonin synthesis) and aromatase. In the present study, the influence of the estrogen status on hypothalamic TPH and serotonin (5-HT) regulation by hypoxia (dissolved oxygen: 1.7 mg/L for 4 weeks) was investigated in croaker. Treatment in vivo with the aromatase inhibitor, ATD (1,4,6-androstatrien-3,17-dione), significantly decreased TPH activity, TPHs (TPH-1 and TPH-2) mRNAs expression, and 5-hydroxytryptophan (5-HTP, an immediate precursor of 5-HT) and 5-HT contents in croaker hypothalamus. Treatment with estradiol-17 beta partially restored hypothalamic TPH activity, TPHs mRNA expression, and 5-HTP and 5-HT contents in hypoxia-exposed fish. These results suggest that the hypoxia-induced inhibition of TPH and 5-HT synthesis is dependent on the estrogen status. To our knowledge, this is the first report of a role for estrogens in modulating neural TPH and 5-HT responses to hypoxia in aquatic vertebrates. (C) 2013 Elsevier Inc. All rights reserved.