Journal
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Volume 162, Issue 2, Pages 134-138Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2009.03.003
Keywords
Organ culture; Testis; Adult zebrafish; Spermatogenesis; Steroidogenesis; Gene expression
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Funding
- CAPES (Brazil)
- Utrecht University (The Netherlands)
- Fundacion Ramon Areces (Spain)
- China Scholarship Council (China)
- Norwegian Research Council (Norway)
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To develop new tools to study the regulation of testis physiology in teleost fish, a medium-term ex vivo organ culture system was adopted for zebrafish testis tissue. The addition of 100 nM 11-ketotestosterone to the system supported complete spermatogenesis, as determined by morphological, molecular and immunohistochemical analyses. Under basal conditions, however, the development of differentiated spermatogonia, spermatocytes, and spermatids was seriously disturbed, probably related to the rapid (within 2 days) down-regulation of the steroidogenic system. Forskolin (0.5 mu M) stimulated acute androgen release from freshly removed tissue and partially prevented down-regulation of the steroidogenic system. The present ex vivo culture system can serve as a tool to evaluate effects of a wide range of substances on the two main functions of the testis, spermatogenesis and hormone production. (C) 2009 Elsevier Inc. All rights reserved.
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