Journal
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Volume 156, Issue 2, Pages 395-409Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2008.01.009
Keywords
Homarus americanus; matrix-assisted laser desorption/ionization-Fourier transform mass spectrometry (MALDI-FTMS); electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF MS/MS); matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS); neuropeptide; neurohormone; peptide sequencing; supraesophageal ganglion; subesophageal ganglion; thoracic ganglia; abdominal ganglia; eyestalk ganglia (ESG); stomatogastric ganglion (STG); sinus gland (SG); pericardial organ (PO); ventral nerve cord (VNC)
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Funding
- NCRR NIH HHS [P20 RR 016463, P20 RR016463] Funding Source: Medline
- NIDDK NIH HHS [R01 DK071801, R01 DK071801-01A1, R01 DK071801-02, R01 DK071801-02S1, 1 R01 DK 071801] Funding Source: Medline
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The American lobster Homarus americanus is a decapod crustacean with both high economic and scientific importance. To facilitate physiological investigations of peptide transmitter/hormone function in this species, we have used matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS), matrix-assisted laser desorption/ionization time-of-flight mass spectrornetry (MALDI-TOF MS) and nanoscale liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry (nanoLC-ESI-Q-TOF MS/MS) to elucidate the peptidome present in its nervous system and neuroendocrine organs. In total, 84 peptides were identified, including 27 previously known H. americanus peptides (e.g., VYRKPPFNGSIFamide [Val(1)-STFamide]), 23 peptides characterized previously from other decapods, but new to the American lobster (e.g., pQTFQYSRGWTNamide [Arg(7)-corazonin]), and 34 new peptides de novo sequenced/detected for the first time in this study. Of particular note are a novel B-type allatostatin (TNWNKFQGSWamide) and several novel FMRFamide-related peptides, including an unsulfated analog of sulfakinin (GGGEYDDYGHLRFamide), two myosuppressms (QDLDHVFLRFamide and pQDLDHVFLRFamide), and a collection of short neuropeptide F isoforms (e.g., DTSTPALRLRFamide and FEPSLRLRFamide). Our data also include the first detection of multiple tachykinin-related peptides in a non-brachyuran decapod, as well as the identification of potential individual-specific variants of orcokinin and orcomyotropin-related peptide. Taken collectively, our results not only expand greatly the number of known H. americanus neuropepticles, but also provide a framework for future studies on the physiological roles played by these molecules in this commercially, and scientifically important species. (C) 2008 Elsevier Inc. All rights reserved.
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